Humanized Green Fluorescent Protein Genes and Methods

Technology #1745

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Researchers
Sergei Zolotukhin
William W. Hauswirth
Nicholas Muzyczka Ph.D.
Managed By
April Kilburn
Assistant Director 352-392-8929

Monitors the Efficiency of Specific Integrated Genes

This synthetic, “humanized” green fluorescent protein (gfph) cDNA can be used as a reporter in gene delivery technologies involving prokaryotes and animals, ideally humans.

Applications

  • Detection of successful gene transfection and translation
  • Testing the efficiency of a particular promoter in any given cell type or tissue

Advantages

  • Small genome and easy real-time product detection, making gfph an ideal reporter for most viral vectors
  • Species independent and does not require any cofactors, substrates, or additional gene products, thus allowing detection in living cells
  • Increased sensitivity (approximately 22-fold for gfph1 and 45-fold for gfph2) over gfp cDNA that has not been “humanized”
  • Can be incorporated into viral and nonviral vector and expression systems

Technology

Scientists at the University of Florida have “humanized” the green fluorescent protein (gfp) cDNA of the jellyfish Aequorea victoria by replacing some of the Aequorea victoria codons with more frequent human codons. The protein that is generated as the cDNA is translated and will allow one to monitor the efficiency of gene transfection and translation when the cDNA is coupled to integrated genes of interest.