This TaqMan-based PCR testing format will allow scientists to quickly, accurately and inexpensively determine the presence of exogenous DNA. It is user-friendly, capable of testing large numbers of samples, and provides a binary readout (a sample is deemed positive or negative for the presence of the target sequences). Employing an internal threshold control (ITC) template, University of Florida researchers simplified PCR testing down to a single reaction. Their improved test overcomes many of the limitations associated with existing assays, reducing the risk of false positives and negatives. Sensitive and specific tests for detecting exogenous DNA molecules are in high demand for infectious disease diagnosis, gene therapy clinical safety trials, and gene doping surveillance programs. This fast, accurate and easy-to-use test is well-positioned in the global PCR market, which is projected to reach $27 billion by 2015.
ApplicationA sensitive and specific test for detecting exogenous DNA molecules
- Collects data automatically and provides fast, accurate, low-cost results within two to three hours
- Analyzes several samples at once, permitting faster processing
- Supports the collection of assay results in centralized databases, facilitating further analysis
- Lowers the risk of false positives or negatives, ensuring accuracy
- Compatible with human and animal DNA analysis, broadening the potential market