Monoclonal Antibodies Targeting Enzyme Involved in Silencing mRNA, Proteins Associated With Liver Cancer, Tumor Growth and Post-Transcription Gene Silencing

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Researchers
Edward Kar Leung Chan
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John Byatt
Assistant Director 352-392-8929

Scientists at the University of Florida have developed a wide variety of monoclonal and polyclonal antibodies, cell lines, vectors and biological materials that can be used in other research projects. Anyone interested in accessing these materials via a license from the University of Florida should contact our office to make arrangements.

Monoclonal Antibody Against Argonaute-2 (Endonuclease Within the RNA-Induced Silencing Complex)

Targets Enzyme Involved in Silencing mRNA

Argonaute-2 (Ago2) is an endonuclease within the RNA-induced silencing complex (RISC) and uses small interfering RNA (siRNA) as a guide to find mRNA containing complementary sequences. Monoclonal antibodies were generated using the full length recombinant human protein as antigen. Journal articles: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1913063/ http://www.plosbiology.org/article/info%3Adoi%2F10.1371%2Fjournal.pbio.1000238

Monoclonal Antibody Against p62/IGF2BP2

Targets Protein that Can Contribute to the Development of Liver Cancer

p62/IGF2BP2 is a cytoplasmic mRNA binding protein which binds to mRNA encoding IGF-II and may be involved in hepatic carcinogenesis because autoantibodies are found in a large percentage of patients with hepatocellular carcinoma. MAb were generated using the full length recombinant human protein as antigen.

Monoclonal Antibody Against p90/CIP2A (gene name KIAA1524)

Targets Protein that Encourages Tumor Growth

p90/CIP2A appears to have been conserved across species, but has no significant homology to any known gene products. It appears to be a companion auto-antigen to IGF2BP2 and represents a new marker for tumors such as hepatocellular carcinoma, gastric cancer and prostate cancer because autoantibodies are found in a large percentage of patients with these cancers. Overexpression of CIP2A has been demonstrated in human head and neck cancer. MAb were generated using the C-terminal third of the recombinant human protein as antigen. Journal article: http://www.nature.com/onc/journal/v21/n32/full/1205625a.html

Monoclonal Antibody Against GW3 (gene name TNRC6C)

Targets a Protein Involved in Post-Transcription Gene Silencing

GW3 is a cellular protein closely related to GW182 protein. GW182 is a critical component of GW bodies, which are small cytoplasmic foci found in proliferating cells and are involved in post-transcriptional regulation of gene expression. MAb were generated using the full length recombinant human GW3 protein as antigen.