Monoclonal Antibodies Against Yeast RNA- and DNA-Binding Proteins (e.g. PUB1, PAB1, NAB2p)

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Maurice Scott Swanson
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John Byatt
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Scientists at the University of Florida have developed a wide variety of monoclonal and polyclonal antibodies, cell lines, vectors and biological materials that can be used in other research projects. Anyone interested in accessing these materials via a license from the University of Florida should contact our office to make arrangements.

PUB1, Targeting Proteins That Aid in RNA Translation and Export

Polyuridylate-binding protein (PUB1) is a member of the ribonuclear protein consensus sequence family of RNA-binding proteins and is structurally related to the human heterologous nuclear RNA-binding proteins (hnRNP) M proteins. PUB1 is localized in a non-uniform pattern throughout both the nucleus and cytoplasm. Antibodies were raised against a preparation of yeast proteins UV crosslinked to polyadenylated RNA. Journal articles: http://mcb.asm.org/content/13/10/6102.long (Molecular and Cell Biology subscription required) http://mcb.asm.org/content/25/13/5499.long (Molecular and Cell Biology subscription required)

PUB 2, Targeting Proteins That Aid in RNA Translation and Export

Polyuridylate-binding protein (PUB2) is also a member of the ribonuclear protein consensus sequence family of RNA-binding proteins and is structurally related to the human heterologous nuclear RNA-binding proteins (hnRNP) M proteins. PUB2 has partially overlapping function with Pub1. Antibodies were raised against a preparation of yeast proteins UV crosslinked to polyadenylated RNA. Journal articles: http://jcb.rupress.org/content/127/5/1173.long (Journal of Cell Biology subscription required) http://www.nature.com/emboj/journal/v21/n7/full/7594399a.html (EMBO Journal subscription required)

Targeting Protein That is Involved in the Construction of mRNA Molecules

Pab1p is a protein which binds poly-A tails of yeast mRNA molecules, and is known as polyA binding protein. It is also known as PAB1, ACBP, CST11 and MRNP and the systematic name is YER165w. It is the only yeast poly-A binding protein, and is found in both the cytoplasm and nucleus of yeast cells. The protein has a molecular weight of 64kDa and antibodies were raised against a preparation of yeast proteins UV crosslinked to polyadenylated RNA. Journal article: http://www.nature.com/emboj/journal/v21/n7/full/7594399a.html (EMBO Journal subscription required)

Targeting a DNA Binding Protein in Yeast

Mammalian protein arginine methyltransferase (PRMT1) is related to a protein arginine methyltransferase (RMT1) in yeast. This same yeast gene has been independently isolated as an hnRNP (heterogeneous nuclear ribonucleoprotein particle) methyltransferase (HMT1). The substrate specificity of recombinant Rmt1p/Hmt1p indicates that this enzyme is a protein methyltransferase I that methylates glycine/arginine-rich domains. Nab1p is one of the “heterogeneous nuclear RNA” or hnRNA binding proteins of yeast. Nab1p is also sometimes referred to as Npl3p, MTR13p, MTS1p and NOP3p and the systematic name is YDR432W. Nab1p is localized in the nucleus where it has a reticuled expression quite different from, for example Nop1p/fibrillarin. The antibody was raised against a preparation of yeast proteins UV crosslinked to polyadenylated RNA. Journal articles: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC19378/ http://jcb.rupress.org/content/127/5/1173.long (Journal of Cell Biology subscription required)

Targeting a RNA Binding Protein That Regulates mRNA Within Cells

Nab2p is a nuclear polyadenylated RNA-binding protein which autoregulates mRNA levels. It is related to human hnRNPs and has nuclear localization signal sequence that binds to Kap104p; required for poly(A) tail length control and nuclear mRNA export. Journal articles: http://www.jbc.org/content/280/26/24532.long (Journal of Biological Chemistry subscription required) http://www.nature.com/emboj/journal/v21/n7/full/7594399a.html (EMBO Journal subscription required) http://jcb.rupress.org/content/127/5/1173.long (Journal of Cell Biology subscription required)

Targeting a DNA Binding Protein That Regulates Production of mRNA

Nab3p is a single stranded DNA binding protein (also known as Hmd1p); acidic ribonucleoprotein; required for termination of non-poly(A) transcripts and efficient splicing; interacts with Nrd1p. The antibody was raised against recombinant Nab3p-maltose binding protein fusion protein. Journal articles: http://jcb.rupress.org/content/127/5/1173.long (Journal of Cell Biology subscription required)

Targeting Proteins That Bind RNA for Gene Expression

Nab4p is a nuclear polyadenylated RNA-binding protein also known as Hrp1p. It is a subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends. The antibody was raised against a Nab4p-GST fusion protein. Journal articles: http://www.jbc.org/content/280/26/24532.long (Journal of Biological Chemistry subscription required) http://www.nature.com/emboj/journal/v17/n24/abs/7591431a.html (EMBO Journal subscription required)