Cell Line Obtained From a Lung Tumor in a Patient With Neurofibromatosis-1
Scientists at the University of Florida have developed a wide variety of monoclonal and polyclonal antibodies, cell lines, vectors and biological materials that can be used in other research projects. Anyone interested in accessing these materials via a license from the University of Florida should contact our office to make arrangements. NF1 Status: The MPNST culture sNF02.2 was derived from tumor material from a 35 year-old male NF1 patient who met diagnostic criteria. The originative tumor tissue for the sNF02.2 cell line was obtained from a lung metastasis diagnosed by histopathology as a MPNST. Cyogenetic analysis and genotyping data is ongoing and will be provided at a later date. The portion of the tumor specimen used for tissue culture was independently characterized by immunohistopathology and confirmed as MPNST. The sNF02.2 tumor culture was established from numerous passages of primary tumor material in culture until they were a homogenous Schwann cell-like population and displayed a clonal morphology immunopositive for both the cytoplasmic Schwann cell markers S100 and p75. Western immunoblotting showed apparently full-length neurofibromin.