Targeting a Gene Product That Mutates to Cause Genetic Disease
Scientists at the University of Florida have developed a wide variety of monoclonal and polyclonal antibodies, cell lines, vectors and biological materials that can be used in other research projects. Anyone interested in accessing these materials via a license from the University of Florida should contact our office to make arrangements. A rabbit polyclonal antibody raised against the neurofibromatosis type 1 (NF1) gene product, neurofibromin. Neurofibromatosis type 1 is a genetic disease with a variety of symptoms that causes neurological tumors. Antiserum was raised against a unique peptide corresponding to peptides 27-41 of the N-terminal sequence of mammalian neurofibromin. Serum was harvested and antibody to neurofibromin was isolated by affinity chromatography against the original peptide. Polyclonal antibody NFn27a binds to human and rodent neurofibromin in various biochemical assays including Western immunoblotting and immunohistochemistry (including paraffin sections). Unlike the other antibodies available to neurofibromin, NF27a does not require antigen retrieval methods for immunolabeling of paraffin sections.