Monoclonal Antibodies Against Heterogeneous Nuclear Ribonuclear Proteins (hnRNP) m3/4 & m1-4

Technology #10452

Questions about this technology? Ask a Technology Manager

Download Printable PDF

Maurice Scott Swanson
Managed By
John Byatt
Assistant Director 352-392-8929

Targeting Proteins That Aid in RNA Translation and Export

Scientists at the University of Florida have developed a wide variety of monoclonal and polyclonal antibodies, cell lines, vectors and biological materials that can be used in other research projects. Anyone interested in accessing these materials via a license from the University of Florida should contact our office to make arrangements. Heterogeneous nuclear ribonucleoproteins (hnRNPs), which directly bind to nascent RNA polymerase II transcripts, play an important role in both transcript-specific packaging and alternative splicing of pre-mRNAs. Datar et al. (1993) isolated and characterized a group of hnRNPs, the M1 to M4 proteins. The M proteins are pre-mRNA-binding proteins in vivo, and they bind avidly to poly(G) and poly(U) RNA homopolymers in vitro. hnRNP-M4 is the largest M protein, with 729 amino acids and a molecular mass of about 77 kD. The M4 protein contains 3 ribonucleoprotein consensus sequence RNA-binding domains of approximately 90 amino acids each, a glycine- and methionine-rich region, and an MR (methionine/arginine) repeat motif. It also contains 9 potential casein kinase type II phosphorylation sites and a tyrosine phosphorylation site. Two-dimensional gels showed multiple M4 isoforms, which may be the result of phosphorylation. This antibody also recognizes hnRNP-M3 as well. Journal article: This antibody recognizes hnRNPs M1 thru M4. Journal article: